Learning Goal: I’m working on a genetics question and need an explanation and answer to help me learn.
please, complete a lab report.
1a) Which of the BIMs show evidence of incorporation of a spacer + repeat into a) the CR1 locus? b) the CR3 locus? 2. Do all BIMs that show the addition of a spacer + repeat only show incorporation into one locus? Post-lab questions 1) Were you successful in isolating BIMs? Give evidence as to why or why not. If not, how would you change the protocol? (For Post-Lab question 1, answer based on “Expt 2 Wk 3 Agarose Gel Images” i.e. your lab period actual data. ) 2) For different BIMs, how would you determine if the same ‘spacer’ sequence was incorporated into each one?3) To prove a BIM had evolved to be phage 2972 resistant, what experiment could you carry out? (Consider the protocol you already carried out and base your answer on this. Indicate the expected results with a ‘BIM’ strain. The agarose gel that needs to be used is GEL#2 Attached is the lab protocol, lab report format, Agarose gel sample data, and agarose gel images
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